Competitive Immunoassay
Real-time quantification of small molecules and haptens in 10 minutes
Overview Competitive assays are ideal for small molecules such as drugs, hormones, toxins and pesticides that are too small for a sandwich format. The sample analyte competes with a labeled or immobilized reagent for limited binding sites, with signal typically decreasing as analyte concentration increases.
ElioDX™ supports different competitive configurations with real-time, wash-free detection and quantitative results in about 10 minutes.
Configurations supported
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Antibody-coated — Sample analyte and labeled antigen compete for an immobilized antibody.
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Antigen-coated — Sample analyte and immobilized antigen compete for a labeled antibody.
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How it works on ElioDX™
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Ready-to-use cuvette — Antibody or antigen is pre-loaded and dried.
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Mix and add — Sample is mixed with the competing reagent and transferred to the cuvette.
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Real-time competition — ElioDX™ continuously monitors the resulting fluorescence signal.
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Quantitative result — Concentration is determined from a calibration curve in about 10 minutes.
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Typical applications
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Drug monitoring and pharmacokinetics
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Hormone quantification
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Mycotoxins, pesticides and residues
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Food and environmental contaminants
Download the Competitive Application Note (PDF) →
Overview Sandwich assays are widely used to detect and quantify larger antigens such as proteins, biomarkers and pathogens. The target is captured between two specific binders, typically a capture and a labeled detection antibody. On ElioDX™, the assay is wash-free and monitored in real time, delivering quantitative results in about 10 minutes from a single sample addition.