Elionova uses a novel photon-counting fluorescence detection system combined with an evanescent field excitation. Unlike traditional ELISA, which relies on enzyme-driven colorimetric reactions and multiple wash steps, Elionova detects binding events in real-time or at endpoint without enzymatic amplification. Only one single step of adding samples, no wash, no incubation. The evanescent field selectively excites only surface-bound fluorophores, eliminating background noise from unbound reagents. This results in faster, more sensitive, and wash-free assays.

Our system uses lasers to generate an evanescent field at the surface of the detection chip, exciting only fluorophores within ~200 nm of the surface. This dramatically reduces background signal. Emitted photons from the labeled molecules are detected by highly sensitive single-photon detectors, enabling precise counting with temporal resolution. Together, this allows quantitative detection of extremely low analyte concentrations with high specificity.

Real-time readout allows continuous signal monitoring, faster time-to-result, kinetic analysis, and simplified assay optimization. It also avoids saturation issues common in endpoint-only formats.

Yes. While real-time detection is available, Elionova’s platform also supports fast, highly sensitive endpoint measurements—ideal for high-throughput and screening applications.

Our assays typically achieve femtomolar to low picomolar sensitivity, with a dynamic range spanning 4–5 orders of magnitude, depending on the target and assay design.

Elionova’s platform is used across research, diagnostics, and industry. Applications include protein and RNA detection, biomarker profiling, antibody screening, and real-time monitoring in fields like food safety, agriculture, bioprocessing, and pharmaceutical R&D. Its speed, sensitivity, and no-wash workflow make it ideal for both laboratory research and applied testing environments.

No — our high-end cuvettes are precisely engineered to generate an evanescent field at the bottom. Each cuvette is individually identified by a unique QR code, readable by the ElioDX Reader.

The reaction at the bottom of the cuvette starts immediately once the sample is added to the wells. Since the Reader performs kinetic measurements based on the initial phase of the reaction, any delay in inserting the cuvette can affect the accuracy of the slope calculation. To ensure consistent and reliable results, the cuvette should be inserted into the Reader within 2 minutes of sample addition.

Yes, all relevant capture components from your established ELISA can be seamlessly transferred to the Elionova Platform. Additionally, we offer comprehensive support with protocols and reagents to enable a streamlined assay setup on our system.

The technology is entirely wash-free — all reagents are pre-dried within the cuvette. The only required step is adding the sample.

ElioDX measures the slope — the rate of signal increase over time. Unlike traditional endpoint measurements, this dynamic approach minimizes the impact of background noise.

Yes, ElioDX Assays deliver quantitative results based on a lot-specific standard curve.

Join one of our demonstrations to learn more.

Yes. ELISA results are typically generated by measuring light intensity, reported in Relative Fluorescence Units (RFU). In contrast, Elionova results are based on photon counting, reported as Counts Per Second (CPS).