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Overview Sandwich assays are widely used to detect and quantify larger antigens such as proteins, biomarkers and pathogens. The target is captured between two specific binders, typically a capture and a labeled detection antibody. On ElioDX™, the assay is wash-free and monitored in real time, delivering quantitative results in about 10 minutes from a single sample addition.

Nucleic Acid Detection

Real-time, wash-free RNA/DNA detection in 10 minutes

Overview ElioDX™ extends beyond immunoassays to oligonucleotide-based nucleic acid detection. Target-specific capture and fluorescent detection probes hybridize with the target sequence, enabling rapid and quantitative detection without antibodies.

How it works on ElioDX™

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Target-specific probes — Capture and fluorescent detection oligos recognize different regions of the target.

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Add the sample — Target nucleic acid hybridizes with both probes.

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Real-time detection — ElioDX™ continuously monitors fluorescence as binding occurs.

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Quantitative result — Concentration is determined from a calibration curve in about 10 minutes.

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Typical applications

  • RNA/DNA quantification

  • Bioprocess and biomass monitoring

  • Fermentation and biomanufacturing QC

  • Custom nucleic acid assays

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Download the Nucleic Acid Detection Application Note (PDF) →

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