Direct Binding & Affinity Screening
Real-time screening of molecular interactions
Overview ElioDX™ enables direct screening of antibody, nanobody and protein interactions, capturing binding continuously in real time rather than at a single endpoint. This allows rapid assessment of binding behavior, specificity and interaction kinetics using a simple, wash-free workflow.
How it works on ElioDX™
​​
Immobilize the target — The target or candidate binder is coated onto the cuvette.
​
Add the binding partner — A labeled interaction partner is added.
​
Real-time monitoring — ElioDX™ continuously tracks binding as it develops.
​
Assess binding — Compare binding profiles, kinetics and specificity.
​
Typical applications
-
Antibody and nanobody screening
-
Protein–protein interaction studies
-
Candidate binder selection
-
Biologics characterization
-
Specificity screening
Download the Binding & Affinity Screening Application Note (PDF) →
Overview Sandwich assays are widely used to detect and quantify larger antigens such as proteins, biomarkers and pathogens. The target is captured between two specific binders, typically a capture and a labeled detection antibody. On ElioDX™, the assay is wash-free and monitored in real time, delivering quantitative results in about 10 minutes from a single sample addition.