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Sandwich Assay

Real-time, quantitative antigen detection in 2-10 minutes

Overview Sandwich assays are widely used to detect and quantify larger antigens such as proteins, biomarkers and pathogens. The target is captured between two specific binders, typically a capture and a labeled detection antibody. On ElioDX™, the assay is wash-free and monitored in real time, delivering quantitative results in about 10 minutes from a single sample addition.

How it works on ElioDX™

 

Ready-to-use cuvette — Capture and detection reagents are pre-loaded and dried.

 

Add the sample — Shake briefly and load the cuvette into the ElioDX™ reader.

 

Real-time detection — Target binding generates a fluorescence signal at the sensor surface.

 

Quantitative result — Results are available within about 10 minutes and accessible via the ElioDX™ digital platform.

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Typical applications

  • Protein biomarkers

  • Pathogen and infectious agent detection

  • Food allergens and contaminants

  • Biomanufacturing quality control

Download the Sandwich Assay Application Note (PDF) →

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